High Electric Production by Membraneless Microbial Fuel Cell with Up Flow Operation Using Acetate Wastewater


  • Aris Mukimin
  • Nur Zen
  • Hanny Vistanty Balai Besar Teknologi Pencegahan Pencemaran Industri
  • Purwanto Agus




ML-MFC, carbon cloth, GDL carbon-Pt, acetate, renewable energy


Microbial fuel cell (MFC) is a new proposed technology reported to generate renewable energy while simultaneously treating wastewater. Membraneless microbial fuel cell (ML-MFC) system was developed to eliminate the requirement of membrane which is expensive and prone to clogging while enhancing electricity generation and wastewater treatment efficiency. For this purpose, a reactor was designed in two chambers and connected via three pipes (1 cm in diameter) to enhance fluid diffusion. Influent flowrate was maintained by adjusting peristaltic pump at the base of anaerobic chamber. Carbon cloth (235 cm2) was used as anode and paired with gas diffusion layer (GDL) carbon-Pt as cathode. Anaerobic sludge was filtered and used as starter feed for the anaerobic chamber. The experiment was carried out by feeding synthetic wastewater to anaerobic chamber; while current response and potential were recorded. Performance of reactor was evaluated in terms of chemical oxygen demand (COD). Electroactive microbe was inoculated from anaerobic sludge and showed current response (0.55-0.65 mA) at 0,35 V, range of diameter 1.5-2 µm. The result of microscopics can showed three different species. The microbial performance was increased by adding ferric oxide 1 mM addition as acceptor electron. The reactor was able to generate current, voltage, and electricity power of 0.36 mA, 110 mV, and 40 mWatt (1.5 Watt/m2), respectively, while reaching COD removal and maximum coulomb efficiency (EC) of 16% and 10.18%, respectively.


Ahn, Y., & Logan, B. E. (2010). Bioresource Technology Effectiveness of domestic wastewater treatment using microbial fuel cells at ambient and mesophilic temperatures. Bioresource Technology, 101(2), 469–475. https://doi.org/10.1016/j.biortech.2009.07.039

Ali, A. E., Gomaa, O. M., Fathey, R., Abd, H., & Kareem, E. (2015). Optimization of double chamber microbial fuel cell for domestic wastewater treatment and electricity production. Journal of Fuel Chemistry and Technology, 43(9), 1092–1099. https://doi.org/10.1016/S1872-5813(15)30032-3

Baranitharan, E., Khan, M. R., Yousuf, A., Fei, W., Teo, A., Yuan, G., & Tan, A. (2015). Enhanced power generation using controlled inoculum from palm oil mill effluent fed microbial fuel cell. FUEL, 143, 72–79. https://doi.org/10.1016/j.fuel.2014.11.030

Barbara, W., & Pawel, W. (2020). The Membrane-Less Microbial Fuel Cell (ML-MFC) with Ni-Co and Cu-B Cathode Powered by the Process Wastewater from Yeast Production. Energies, 13153976(1), 1–13.

Bhargavi, G., Venu, V., & Renganathan, S. (2018). Microbial fuel cells : recent developments in design and materials Microbial fuel cells : recent developments in design and materials. In Materials Science and Engineering 330 (p. 012034). https://doi.org/10.1088/1757-899X/330/1/012034

Bond, D. R., Lovley, D. R., Bond, D. R., & Lovley, D. R. (2003). Electricity Production by Geobacter sulfurreducens Attached to Electrodes Electricity Production by Geobacter sulfurreducens Attached to Electrodes, 69(3). https://doi.org/10.1128/AEM.69.3.1548

Di, M., Curtis, T. P., Head, I. M., & Scott, K. (2009). A single-chamber microbial fuel cell as a biosensor for wastewaters. Water Research, 43(13), 3145–3154. https://doi.org/10.1016/j.watres.2009.01.005

Feng, Y., Wang, X., Logan, B. E., & Lee, H. (2008). Brewery wastewater treatment using air-cathode microbial fuel cells, 873–880. https://doi.org/10.1007/s00253-008-1360-2

Fornero, J. J., Rosenbaum, M., & Angenent, T. (2010). Electric Power Generation from Municipal , Food , and Animal Wastewaters Using Microbial Fuel Cells. https://doi.org/10.1002/elan.200980011

Greenman, J., Gálvez, A., Giusti, L., & Ieropoulos, I. (2009). Enzyme and Microbial Technology Electricity from landfill leachate using microbial fuel cells : Comparison with a biological aerated filter, 44, 112–119. https://doi.org/10.1016/j.enzmictec.2008.09.012

Jadhav, G. S., & Ghangrekar, M. M. (2009). Bioresource Technology Performance of microbial fuel cell subjected to variation in pH , temperature , external load and substrate concentration. Bioresource Technology, 100(2), 717–723. https://doi.org/10.1016/j.biortech.2008.07.041

Kim, J., Kim, B., An, J., Lee, Y. S., & Chang, I. S. (2016). Bioresource Technology Development of anode zone using dual-anode system to reduce organic matter crossover in membraneless microbial fuel cells. BIORESOURCE TECHNOLOGY, 213, 140–145. https://doi.org/10.1016/j.biortech.2016.03.012

Larrosa-guerrero, A., Scott, K., Head, I. M., Mateo, F., Ginesta, A., & Godinez, C. (2010). Effect of temperature on the performance of microbial fuel cells. Fuel, 89(12), 3985–3994. https://doi.org/10.1016/j.fuel.2010.06.025

Li, Z., Zhang, X., Lin, J., Han, S., & Lei, L. (2010). Bioresource Technology Azo dye treatment with simultaneous electricity production in an anaerobic – aerobic sequential reactor and microbial fuel cell coupled system. Bioresource Technology, 101(12), 4440–4445. https://doi.org/10.1016/j.biortech.2010.01.114

Liu, Z., & Li, H. (2007). Effects of bio- and abio-factors on electricity production in a mediatorless microbial fuel cell, 36, 209–214. https://doi.org/10.1016/j.bej.2007.02.021

Pant, D., Bogaert, G. Van, Diels, L., & Vanbroekhoven, K. (2010). Bioresource Technology A review of the substrates used in microbial fuel cells ( MFCs ) for sustainable energy production. Bioresource Technology, 101(6), 1533–1543. https://doi.org/10.1016/j.biortech.2009.10.017

Reimers, C. E., Iii, H. A. S., Westall, J. C., Alleau, Y., Howell, K. A., Soule, L., … Girguis, P. R. (2007). Substrate Degradation Kinetics , Microbial Diversity , and Current Efficiency of Microbial Fuel Cells Supplied with Marine Plankton Substrate Degradation Kinetics , Microbial Diversity , and Current Efficiency of Microbial Fuel Cells Supplied with Marine Plankton ᰔ. https://doi.org/10.1128/AEM.01209-07

Roma, B. (2008). Importance of temperature and anodic medium composition on microbial fuel cell ( MFC ) performance, 1213–1218. https://doi.org/10.1007/s10529-008-9687-4

Santoro, C., Arbizzani, C., Erable, B., & Ieropoulos, I. (2017). Microbial fuel cells : From fundamentals to applications . A review. Journal of Power Sources, 356, 225–244. https://doi.org/10.1016/j.jpowsour.2017.03.109

Sun, J., Li, W., Li, Y., Hu, Y., & Zhang, Y. (2013). Bioresource Technology Redox mediator enhanced simultaneous decolorization of azo dye and bioelectricity generation in air-cathode microbial fuel cell. Bioresource Technology, 142, 407–414. https://doi.org/10.1016/j.biortech.2013.05.039

Tartakovsky, B., & Guiot, S. R. (2006). A Comparison of Air and Hydrogen Peroxide Oxygenated Microbial Fuel Cell Reactors, (Figure 1), 241–246.

Thung, W., Ong, S., Ho, L., Wong, Y., Ridwan, F., & Oon, Y. (2015). Bioresource Technology A highly efficient single chambered up-flow membrane-less microbial fuel cell for treatment of azo dye Acid Orange 7-containing wastewater. BIORESOURCE TECHNOLOGY, 197, 284–288. https://doi.org/10.1016/j.biortech.2015.08.078

Wang, X. (2014). Production of Electricity during Wastewater Treatment Using Fluidized-Bed Microbial Fuel Cells, (4), 703–708. https://doi.org/10.1002/ceat.201300241

Wardana, K., & Effendi, A. (2019). The Concentration Variation of Wastewater from Pulp Washing Process in Membraneless Air Cathode Microbial Fuel Cell. Jurnal Selulosa, 9(2), 75–86.

Wei, L., Han, H., & Shen, J. (2012). Effects of cathodic electron acceptors and potassium ferricyanide concentrations on the performance of microbial fuel cell. International Journal of Hydrogen Energy, 37(17), 12980–12986. https://doi.org/10.1016/j.ijhydene.2012.05.068

Zhu, F., Wang, W., Zhang, X., & Tao, G. (2011). Bioresource Technology Electricity generation in a membrane-less microbial fuel cell with down-flow feeding onto the cathode. Bioresource Technology, 102(15), 7324–7328. https://doi.org/10.1016/j.biortech.2011.04.062

Zhuwei, D. U., Qinghai, L. I., Meng, T., & Shaohua, L. I. (2008). Electricity Generation Using Membrane-less Microbial Fuel Cell during Wastewater Treatment *. Chinese Journal of Chemical Engineering, 16(5), 772–777. https://doi.org/10.1016/S1004-9541(08)60154-8



How to Cite

Mukimin, A., Zen, N., Vistanty, H., & Agus, P. (2020). High Electric Production by Membraneless Microbial Fuel Cell with Up Flow Operation Using Acetate Wastewater. Jurnal Riset Teknologi Pencegahan Pencemaran Industri, 11(2), 19-27. https://doi.org/10.21771/jrtppi.2020.v11.no2.p19-27



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